The Impact of Long-Term Feeding of Dietary Jojoba Oil and Cholesterol on the HDL Cholesterol Concentration and the Development of Atherosclerosis in New Zealand White Rabbits

Authors:

Tarek Karam, Anahis Rincon

Mentor:

Raymond Garcia, Research Advisor, California State University, Los Angeles

Dietary Jojoba seed oil has been shown to maintain high-density lipoprotein cholesterol (HDL-C) concentration at an elevated level in hypercholesterolemic New Zealand White (NZW) rabbits, suggesting that jojoba oil regulates HDL metabolism and supposedly has a protective effect against atherosclerosis, one of the leading causes of death worldwide. We hypothesize that jojoba oil decreases the development of atherosclerosis in cholesterol fed NZW rabbits. In this study, NZW rabbits were fed either a normal chow (N) diet, or one supplemented with either 3% jojoba seed oil (J), 1% cholesterol (C), or 1% cholesterol + 3% jojoba seed oil (CJ) for 9 weeks. Throughout the study, HDL-C was isolated from serum by polyanion precipitation, and HDL-C concentrations were monitored via an enzymatic cholesterol assay. Rabbits were euthanized at week 9 and aortas were extracted and assessed for atherosclerosis. There were no statistical difference in HDL-C concentrations for the N- and J-fed rabbits and no development of atherosclerotic lesions in those animals, as was expected. C-fed rabbits had a lower HDL-C concentration (7.56 mg/dL) with significant atherosclerotic lesion development, while CJ-fed rabbits had a higher HDL-C concentration (18.04 mg/dL) and a higher development of atherosclerotic lesions than those fed the C diet. The results indicate that there were more atherosclerotic lesions in the CJ-fed rabbit than in the C-fed rabbit, which was not expected. More experiments will be needed to validate these results, which will provide a better understanding of how dietary jojoba seed oil, in the presence of dietary cholesterol, regulates HDL metabolism and the development of atherosclerosis. (CSU-LSAMP is supported by the National Science Foundation under Grant # HRD-0802628 and the CSU Office of the Chancellor.)


Presented by:


Date:

Saturday, November 23, 2013

Poster:

36

Room:

Poster Session 3 - Villalobos Hall

Presentation Type:

Poster Presentation

Discipline:

Biochemistry