Investigation of the Mode of Action of a Novel Anti-Ethylene and Anti-Auxinic Compound in Plants
Authors:n/a n/a, Alicia Vazquez
Mentor:Paul Larsen, Investigation of the Mode of Action of a Novel Anti-Ethylene and Anti-Auxinic Compound in Plants, UC Riverside
Investigation of the Mode of Action of a Novel Anti-Ethylene and Anti-Auxinic Compound in Plants Author: Alicia Vazquez (CSU Channel Islands) Mentor: Dr. Paul B. Larsen (Department of Biochemistry, UC Riverside) Abstract: Previous research has found that auxin and ethylene work synergistically together. Auxin has been shown to regulate ethylene dependent apical hook formation, root growth, and hypocotyl length. However, it is not completely understood how the ethylene triple response phenotype is controlled by auxin. Through a chemical library screen, an active compound that inhibits the ethylene dependent triple response phenotype was isolated and studied to better comprehend the manner in which ethylene signal transduction occurs and is regulated by other hormones. It was shown that in the presence of the active compound, the ethylene triple response phenotype no longer occurred. This included agravitropic roots and no apical hook formation. A GC analysis for ethylene was conducted to eliminate the possibility of differences in the amount of ethylene biosynthesis. Because no significant differences between the amounts of ethylene produced were detected, the experiments that followed focused on the ethylene regulating hormone auxin. Col-0 wt Arabidopsis were germinated in increasing amounts of the active compound and after several days hook formation, hypocotyl and root length were measured to clearly show the effects of the active compound. In addition, Col-0 wt seedlings were exposed to both the active compound and auxin to prove if it is either inhibiting response or production of auxin; the results supported inhibition. To further reinforce the theory of inhibition, a DR5:GUS staining was conducted to physically see if and/or where auxin was present when exposed to the active compound; auxin was detected in all DR5:GUS samples. From the results of the GC, DR5:GUS staining and growth analyses, it was concluded that the active compound is anti-auxinic.