Effects of a Collagen Scaffold on Mouse Bone Marrow Stromal (S17) Cell Culture
Mentor:Chad Barber, Assistant Professor of Biology, California Lutheran University
Currently, cell culture is commonly performed on rigid, two-dimensional plastic dishes. Traditional cell culture is not representative of the flexible, three-dimensional (3-D) microenvironment found in vivo. Cells respond very differently when placed on a 3-D matrix in culture made out of any number of natural or synthetic polymer molecules. Many cell lines have been shown to grow better in 3-D culture; however, cells from the mouse bone marrow stromal cell line, S17, have not been studied in the context of a 3-D culture system. Bone marrow stromal cells may prove invaluable in maintaining the blood generating, hematopoietic stem cells (HSCs), whose culture conditions have not been fully elucidated. Current aims are to develop a 3-D culture system in which S17 cells can survive, proliferate, and support HSCs. The effects of collagen gel thickness and elasticity on cell growth and survival were studied. S17 cells had improved growth on a more flexible 3-D environment, with an average of 2.46 x 10^6 cells/mL in 2-D as compared to an average of 2.99 x 10^6 cells/mL in a 3-D collagen matrix. Experimentation will show if S17 cells prefer a specific concentration of collagen when compared to various other concentrations. Future work includes culturing mouse hematopoietic stem cells on a collagen scaffold as well as culturing both cell types on polyacrylamide hydrogels. Also, a combination of hematopoietic stem cells and S17 cells on a 3-D matrix could also be used to recreate a bone marrow environment for the cells.