Comparison between chorion proteins of Latrodectus hesperus and Latrodectus geometricus.
Mentor:Merri Casem, Professor of Biology, California State University, Fullerton
A chorion is the outermost membrane of an embryo in most animals. In insects it is usually considered the shell of the egg. The chorion is responsible for keeping the nutrients and the embryo safe and sound. Chorion proteins of Latrodectus hesperus and Latrodectus geometricus were isolated from in-lab laid eggs. The experiment was performed in order to compare the chorion composition of both species. between the to species, fertilized and unfertilized and/or a difference in the proteins regarding the development of the eggs. We ran various electrophoresis gels to determine any differences or similarities. We anticipated, to see somewhat of a difference between the two species, and our results indicated that subtly different proteins composed the chorions of the different species. Our results showed a difference between early stages and later stage of egg development as well. Future research might include the correct identification of what kind of proteins the chorions are composed of by proteomics. Overall, these results can be applied to other spider chorion research.