Analysis of the effects of ionic manganese on Caenorhabditis elegans
Authors:Xiao Chen, Martha Harper, Joshua Hwang, Kenneth Kang, Jenae Melendez
Mentor:Chandra Srinivasan, Associate Professor Biochemistry, California State University Fullerton
Analysis of the effects of ionic manganese on Caenorhabditis elegans Xiao Chen1, Jenae Melendez1, Kenneth Kang1, Martha Harper1, Joshua Hwang2, and Chandra Srinivasan1. 1 California State University Fullerton. 2 University of Southern California. Manganese (Mn) is an essential trace mineral that is involved in numerous biological processes due to its ability to break down reactive oxygen species (ROS), a process better known as antioxidant like activity. Yet, the process by which Mn scavenges free radicals (antioxidant) remains unclear. Our hypothesis is that Mn, along with regulating cellular protective factors will form small molecule complexes with metabolites to neutralize ROS in a cell. Caenorhabditis elegans (C. elegans) a free-living round worm was used as a model system to test this hypothesis. Inductively coupled plasma mass spectrometry (ICP-MS) was used to measure Mn concentration in the ultra filtrates. C. elegans, were grown on agar plates in the presence or absence of Mn supplementation. Based on seven independent trials, there was a ten-fold increase in Mn levels in the ultrafiltrates obtained from worms grown on Mn-enriched diet, in comparison to worms grown on a normal diet. The normal diet in this instance consisted of Escherichia coli (E. coli) or OP50-1 on a agar plate without Mn. The ultrafiltrates prepared were devoid of DNA and protein by gel electrophoresis techniques. Antioxidant capacity was also examined in vitro via neutralization of hydrogen peroxide and pro-oxidant, with a fluorogenic probe 2’7’-dichlorofluorescein (DCFH). Learning more about the molecular composition of the ultrafiltrates might provide insights into the Mn-mediated mechanism of ROS scavenging in C. elegans and will in turn provide insight on how manganese will benefit humans. These benefits include but are not limited to as reduce the chances of heart disease, reduces the affects of ROS within the body, and even prevent certain cancers.